Fungi as Allies in Environmental Remediation: A Global Bibliometric Assessment of Mycoremediation Research
Jayzon G. Bitacura
Abstract:
Background: Mycoremediation, the use of fungi to degrade,
transform, or immobilize environmental pollutants, has
emerged as a sustainable and innovative environmental
remediation strategy. Understanding global research trends in
this field is essential to identify scientific progress, research
gaps, and emerging directions.
Objectives: To evaluate the global research landscape, trends,
and emerging themes in mycoremediation through a
bibliometric analysis of Scopus-indexed publications.
Methods: A bibliometric analysis was conducted using the
Scopus database, utilizing 907 documents related to
mycoremediation. Bibliometric mapping was performed using
Scopus Analyze, Bibliometrix, and VOSviewer to examine
publication trends, document types, subject areas, journals,
authors, institutions, countries, and thematic evolution.
Results: Research activity was limited between 1976 and the
early 2000s, with fewer than ten publications annually. Growth
accelerated after 2010 and increased significantly after 2015,
reaching more than 125 publications in 2024. Journal articles
dominated the dataset, reflecting strong interdisciplinarity
across environmental science, microbiology, agriculture,
biochemistry, and engineering. Chemosphere emerged as the
leading journal, while highly cited studies focused on fungal
biosorption, hydrocarbon degradation, and plastic
biodegradation. Authorship and country analyses identified
India, Europe, and the United States as key contributors and
central collaborators. Thematic evolution revealed a shift from
early research on ligninolytic enzymes and hydrocarbons to
emerging topics such as heavy metals, dye effluents, plastics,
and pharmaceuticals.
Conclusion: Mycoremediation research has expanded rapidly
and is increasingly recognized as an ecologically relevant
solution to environmental pollution. However, gaps remain,
including limited field-scale applications, underutilization of
omics technologies, and low research representation from
biodiversity-rich regions. Future work should integrate
molecular tools, strengthen global collaboration, and translate
laboratory findings into scalable environmental applications.
References:
- Ahmed, I., Zia, M. A., Afzal, H., Ahmed, S., Ahmad, M., Akram, Z., Sher, F., & Iqbal, H. M. N. (2021). Socio-economic and environmental impacts of biomass valorisation: A strategic drive for sustainable bioeconomy. Sustainability, 13(8), Article 4200. https://doi.org/10.3390/su13084200
- Akhtar, N., & Mannan, M. A.-U. (2020). Mycoremediation: Expunging environmental pollutants. Biotechnology Reports, 26, Article e00452. https://doi.org/10.1016/j.btre.2020.e00452
- Ali, Z., Abdullah, M., Hafeez, F., Alasmari, A., Sardar, F., Ahmad, J., Ahmad, F., Alam, M., & Yasin, M. T. (2025). Advances in myco-remediation strategies for emerging contaminants: A comprehensive review. Bioremediation Journal, 1–37. https://doi.org/10.1080/10889868.2025.2507431
- Aranda, E. (2016). Promising approaches towards biotransformation of polycyclic aromatic hydrocarbons with Ascomycota fungi. Current Opinion in Biotechnology, 38, 1–8. https://doi.org/10.1016/j.copbio.2015.12.002
- Aria, M., & Cuccurullo, C. (2017). bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics, 11(4), 959–975. https://doi.org/10.1016/j.joi.2017.08.007
- Azubuike, C. C., Chikere, C. B., & Okpokwasili, G. C. (2020). Bioremediation: An eco-friendly sustainable technology for environmental management. In G. Saxena & R. N. Bharagava (Eds.), Bioremediation of Industrial Waste for Environmental Safety: Volume I: Industrial Waste and Its Management (pp. 19–39). Springer. https://doi.org/10.1007/978-981-13-1891-7_2
- Baldrian, P. (2003). Interactions of heavy metals with white-rot fungi. Enzyme and Microbial Technology, 32(1), 78–91. https://doi.org/10.1016/S0141-0229(02)00245-4
- Bartolomeu, M., Neves, M. G. P. M. S., Faustino, M. A. F., & Almeida, A. (2018). Wastewater chemical contaminants: Remediation by advanced oxidation processes. Photochemical & Photobiological Sciences, 17(11), 1573–1598. https://doi.org/10.1039/C8PP00249E
- Bashir, I., Lone, F. A., Bhat, R. A., Mir, S. A., Dar, Z. A., & Dar, S. A. (2020). Concerns and threats of contamination on aquatic ecosystems. In K. R. Hakeem, R. A. Bhat, & H. Qadri (Eds.), Bioremediation and Biotechnology: Sustainable Approaches to Pollution Degradation (pp. 1–26). Springer International Publishing. https://doi.org/10.1007/978-3-030-35691-0_1
- Bitacura, J. G., Balala, A. C., & Abit, P. P. (2012). Fungi from coastal sediments as potential agents in biodegrading used engine oil. Annals of Tropical Research, 34(2), 112–125. https://doi.org/10.32945/atr3427.2012
- Bitacura, J. G., & dela Cruz, T. E. E. (2026). Enhanced production of ligninolytic enzymes by mangrove fungal endophytes co-cultured with pathogenic and beneficial fungi. Asian Journal of Mycology, 9(1), 1–19. https://doi.org/10.5943/ajom/9/1/1
- Bitacura, J. G., & Santos, M. D. (2023). In silico structural analysis, classification, and functional annotation of an uncharacterized protein from an aquatic fungus Lindgomyces ingoldianus. Genetics of Aquatic Organisms, 7(1), GA527. https://doi.org/10.4194/GA527
- Bitacura, J. G., Jacob, J. K. S., & dela Cruz, T. E. E. (2024). Isolation and identification of fungal endophytes associated with leaves of Rhizophora mucronata Lamk. Acta Manilana, 72, 1–17. https://doi.org/10.53603/actamanil.72.2024.sger2382
- Bonnarme, P., & Jeffries, T. W. (1990). Mn(II) regulation of lignin peroxidases and manganese-dependent peroxidases from lignin-degrading white rot fungi. Applied and Environmental Microbiology, 56(1), 210–217. https://doi.org/10.1128/aem.56.1.210-217.1990
- Donthu, N., Kumar, S., Mukherjee, D., Pandey, N., & Lim, W. M. (2021). How to conduct a bibliometric analysis: An overview and guidelines. Journal of Business Research, 133, 285–296. https://doi.org/10.1016/j.jbusres.2021.04.070
- Eliseo, R. M. M., & Bitacura, J. G. (2021). Heavy metal tolerance of filamentous fungi from the sediments of Visayas State University wastewater pond. Annals of Tropical Research, 43(1), 88–101. https://doi.org/10.32945/atr4317.2021
- Elsevier. (2025). Scopus: Analyze search results tool [Database / analytics tool]. https://www.scopus.com
- Ghosal, D., Ghosh, S., Dutta, T. K., & Ahn, Y. (2016). Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): A review. Frontiers in Microbiology, 7, 1369. https://doi.org/10.3389/fmicb.2016.01369
- Gunyar, O. A., & Uztan, A. H. (2021). Environmental mycobiotechnology in special reference to fungal bioremediation. In N. Saglam, F. Korkusuz, & R. Prasad (Eds.), Nanotechnology Applications in Health and Environmental Sciences (pp. 361–383). Springer International Publishing. https://doi.org/10.1007/978-3-030-64410-9_20
- Hüttel, W., & Hoffmeister, D. (2011). Fungal biotransformations in pharmaceutical sciences. In M. Hofrichter (Ed.), Industrial applications (The Mycota, Vol. 10). Springer. https://doi.org/10.1007/978-3-642-11458-8_14
- Irfan, M., Almotiri, A., & AlZeyadi, Z. A. (2022). Antimicrobial resistance and its drivers—A review. Antibiotics, 11(10), 1362. https://doi.org/10.3390/antibiotics11101362
- Jalil, M. T. M. (2025). Sustainable approaches mitigate environmental pollution. In A. Z. Yaser, M. A. Abu Samah, F. Ariffin, & A. K. Haghi (Eds.), Controlling Environmental Pollution: Practical Solutions (pp. 163–195). Springer Nature. https://doi.org/10.1007/978-981-97-8931-3_9
- Kapoor, A., & Viraraghavan, T. (1995). Fungal biosorption—An alternative treatment option for heavy metal bearing wastewaters: A review. Bioresource Technology, 53(3), 195–206. https://doi.org/10.1016/0960-8524(95)00072-M
- Kapoor, A., Viraraghavan, T., & Cullimore, D. R. (1999). Removal of heavy metals using the fungus Aspergillus niger. Bioresource Technology, 70(1), 95–104. https://doi.org/10.1016/S0960-8524(98)00192-8
- Kuppusamy, S., Palanisami, T., Megharaj, M., Venkateswarlu, K., & Naidu, R. (2016). Ex-situ remediation technologies for environmental pollutants: A critical perspective. In P. de Voogt (Ed.), Reviews of Environmental Contamination and Toxicology, Volume 236 (pp. 117–192). Springer International Publishing. https://doi.org/10.1007/978-3-319-20013-2_2
- Macaskie, L. E. (1990). An immobilized cell bioprocess for the removal of heavy metals from aqueous flows. Journal of Chemical Technology & Biotechnology, 49(4), 357–379. https://doi.org/10.1002/jctb.280490408
- Manguilimotan, L. C., & Bitacura, J. G. (2018). Biosorption of cadmium by filamentous fungi isolated from coastal water and sediments. Journal of Toxicology, 2, 7170510. https://doi.org/10.1155/2018/7170510
- Maqbool, Z., Hussain, S., Imran, M., Mahmood, F., Shahzad, T., Ahmed, Z., Azeem, F., & Muzammil, S. (2016). Perspectives of using fungi as bioresource for bioremediation of pesticides in the environment: A critical review. Environmental Science and Pollution Research, 23(17), 16904–16925. https://doi.org/10.1007/s11356-016-7003-8
- Mishra, M., Prasad, R., & Varma, A. (2015). Endophy fungi: Biodiversity and functions. International Journal Pharma and Bio Sciences, 6(1), B18–B36. https://www.ijpbs.net/counter.php?aid=3913
- Muhonja, C. N., Makonde, H., Magoma, G., & Imbuga, M. (2018). Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi-Kenya. PLOS ONE, 13(7), e0198446. https://doi.org/10.1371/journal.pone.0198446
- Paszczynski, A., & Crawford, R. L. (1995). Potential for bioremediation of xenobiotic compounds by the white-rot fungus Phanerochaete chrysosporium. Biotechnology Progress, 11(4), 368–379. https://doi.org/10.1021/bp00034a002
- Sharma, S., Tiwari, S., Hasan, A., Saxena, V., & Pandey, L. M. (2018). Recent advances in conventional and contemporary methods for remediation of heavy metal-contaminated soils. 3 Biotech, 8(4), 216. https://doi.org/10.1007/s13205-018-1237-8
- Singh, R. L., & Singh, P. K. (2017). Global environmental problems. In R. L. Singh (Ed.), Principles and Applications of Environmental Biotechnology for a Sustainable Future (pp. 13–41). Springer. https://doi.org/10.1007/978-981-10-1866-4_2
- Solanki, S., Sinha, S., & Singh, R. (2022). Myco-degradation of microplastics: An account of identified pathways and analytical methods for their determination. Biodegradation, 33(6), 529–556. https://doi.org/10.1007/s10532-022-10001-6
- Sosa-Martínez, J. D., Balagurusamy, N., Montañez, J., Peralta, R. A., Moreira, R. de F. P. M., Bracht, A., Peralta, R. M., & Morales-Oyervides, L. (2020). Synthetic dyes biodegradation by fungal ligninolytic enzymes: Process optimization, metabolites evaluation and toxicity assessment. Journal of Hazardous Materials, 400, 123254. https://doi.org/10.1016/j.jhazmat.2020.123254
- Suryadi, H., Judono, J. J., Putri, M. R., Eclessia, A. D., Ulhaq, J. M., Agustina, D. N., & Sumiati, T. (2022). Biodelignification of lignocellulose using ligninolytic enzymes from white-rot fungi. Heliyon, 8(2), e08865. https://doi.org/10.1016/j.heliyon.2022.e08865
- van Eck, N. J., & Waltman, L. (2009). Software survey: VOSviewer, a computer program for bibliometric mapping. Scientometrics, 84(2), 523–538. https://doi.org/10.1007/s11192-009-0146-3
- Winquist, E. (2014). The potential of ligninolytic fungi in bioremediation of contaminated soils. Aalto University. https://aaltodoc.aalto.fi/handle/123456789/13003
- Yarzábal Rodríguez, L. A., Álvarez Gutiérrez, P. E., Gunde-Cimerman, N., Ciancas Jiménez, J. C., Gutiérrez-Cepeda, A., Ocaña, A. M. F., & Batista-García, R. A. (2024). Exploring extremophilic fungi in soil mycobiome for sustainable agriculture amid global change. Nature Communications, 15(1), 6951. https://doi.org/10.1038/s41467-024-51223-x
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