Home⇛The Philippine Journal of Biochemistry and Molecular Biology (PJBMB)⇛vol. 11 and 12 no. 1 (1993)

Determination, Localization, and Partial Characterization of Natural Antioxidants in Musaea

Joy Bartolome Duldulao | Evelyn Mae T. Mendoza | Antonio C. Laurena

Discipline: agriculture, food and related studies

 

Abstract:

Analysis of the antioxidative activity of six banana varieties (Bungulan, Lakatan, Latundan, Pisang walung, Pundol and Saba), abaca (Musa textilis) and Heliconia by the thiobarbituric and thiocyanate tests showed that Lakatan, abaca and Pundol had appreciable activity in the leaf. This was observed in both chloroform and 80% ethanol extracts as well as in both young and mature leaves. The ethanol extract of the pseudostem of abaca showed the highest antioxidant activity compared to the corm, leaf and bract of two banana varieties and abaca. High pressure liquid chromatography of the abaca pseudostem ethanol extract showed seven peaks. Antioxidant activity was located in the second peak. Thin layer chromatography of the active peak gave two bands with Rf Values of 0.12 and 0.65. Partial characterization of these bands using chromatography revealed that the Rf 0.65 constituent was phenolic and the Rf 0.12, nonphenolic. The band of Rf 0.65 corresponded to chlorogenic acid.



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