Effect of Ascorbic Acid on Saccharin-enhanced Mutagenicity of Mitomycin C

Leandro  Dimayuga,; Julius  Dalupang,; Chona  Diokno,; Raymundo  Dimaculangan,; H.v.  Barcelon,; A.o.  Cantillep,; M.t.  Cortes,; J.f.  David,; G.a.  Del Castillo,; M.v.  Demetria,; Dalisay  Sy,

Effect of Ascorbic Acid on Saccharin-enhanced Mutagenicity of Mitomycin C

Discipline: Biological and Sport Sciences

Abstract:

Effectiveness of ascorbic acid in inhibiting the enhancing effect of saccharin on the mutagenicity of mitomycin C was demonstrated. Eight to nine weeks old Strong A male mice were injected i.p. with mitomycin C at a concentration of 3 mg/Kg BW. This was followed 3h after by i.p. injection of saccharin, 2.5 g/Kg BW. Two groups of experimental animals were utilized: one, receiving ascorbic acid lh after saccharin injection and the second group, lh before administration of saccharin. Both groups were sacrificed 30h after mitomycin C treatment and bone marrow cells isolated following Schmid's procedure. Cells were smeared, stained with May Grunwald Giemsa and scored for micronuclei. Animals that received ascorbic acid prior to saccharin treatment showed 48% reduction in the number of micronuclei. In contrast, animals that received the vitamin after saccharin injection showed 3.87% reduction. Results suggest the effectivity of ascorbic acid in diminishing the effect of mitomycin C when the vitamin is given before but not after saccharin administration.

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