Discipline: Veterinary Medicine
The estrogenic effects of Sida cordifolia L., locally-known as "gulipas'' on vaginal opening and cytology, uterine and glandular-epithelial development, and blood and uterine eosinophil count were examined in immature female Sprague-Dawley rats. Sida cordifolia incorporated in the diet, was given to intact and ovariectomized (OVX) immature rats (5% S. cordifolia: Intact and OVX groups-5%; 10% S. cordifolia; Intact and OVX groups-10%) for 30 days. Intact and OVX female rats were used as control (basal diet: Intact and OVX groups-B; 17β-estradiol Intact and OVX groups-E). Dietary incorporation of S. cordifolia in OVX rats caused a significant increase in the uterine weight (p<0.05). OVX group-5% and -10% S. cordifolia showed complete vaginal opening and noncyclic leukocytic vaginal smear pattern. However, blood eosinophil count and uterine development were not significantly different in the plant-fed groups. Eosinophilic infiltration of the uterus was also not evident. Based on these findings, S. cordifolia appeared to contain estrogen-like activity which could have induced an increase in uterine weight as well as induced vaginal opening in the plant-fed rats. However, these estrogen-like properties appear insufficient to cause peripheral eosinophilia as well as eosinophilic uterine infiltration.