HomePhilippine Journal of Veterinary Medicinevol. 43 no. 2 (2006)

Isolation, Serologic Identification and Antibiotic Sensitivity Testing of Salmonella spp. in Chickens

Lotis M. Balala | Hope G. Rovira | Maria Fe C. Vizmanos | Francis Andrew Eugene M. Bernardo | Billy P. Divina

Discipline: Veterinary Medicine



The present study aimed to isolate Salmonella from chickens and identify the serotypes and determine the antibiotic sensitivity patterns of the isolates. Meats and eggs were obtained from two local public markets whereas cloacal swabs were collected from two broiler and two layer farms. Isolation and serotyping of Salmonella were done by using conventional method and specific typing sera, respectively. Antibiotics sensitivity test was carried out by the Kirby-Bauer method. Of the 325 samples, 16 (4.9%) contained Salmonella. These consisted of 9.3% out of 150 meat and 2.0% out of 100 cloacal swab samples. Salmonella was not detected in 75 pooled egg samples . There was no significant difference in the recovery rates of Salmonella based on the type of meat sampled. Seven serotypes were found with S. weltevreden predominating, followed by S. derby, S. enteritidis PT1, S. enteritidis phage type untypable, S. newport and S. lexington. S. albany was isolated locally for the first time. All isolates were sensitive to norfloxacin, gentamicin, cephalothin but were resistant to nitro furantoin. All serotypes , were also resistant to at least one antibiotic while 9.7% showed multi-drug resistance to nitrofurantoin, tetracyline and trimethoprim-sulfamethoxazole. This study reveals that Salmonella continues to contaminate our food with new serotypes present including S. enteritidis PT1 and S. albany. The antibiotic sensitivity patterns of Salmonella serotypes have changed and showed particular resistance to nitrofurantoin. Therefore, it is recommended that proper food handling practices be emphasized repeatedly and that antibiotics be prudently used in food animals.