Philippine E-Journals

Dt. Diaphorase Polymorphism In A Natural Population Of Rattus Rattus Mindanensis

Rita P. Laude | Dolores Ramirez

 

Abstract:

DT-diaphorase polymorphism was detected in a sample of 128 Rattus rattus mindanensis collected at r8.!),dom from farmers in the towns of Bay, Lumban, Pakil, Pangil, and Mabitac in Laguna, Philippines. Crude liver extract and hemolysates of rats treated subcutaneously with 0.0005% warfarin were used. Both liver and erythrocyte DT-diaphorase activity were highly variable indicating 3 wide phenotypic variability. The average level of liver DT-diaphorase activity observed is near the mean value of heterozygous resistant Rattus noruegic~ DT-diaphorCJ~~ isozyme polymorphism was found in liver and erythrocyte using disc-gel electrophoresis. Fifteen DT-diaphorase isozymes were resolved with crude extract from liver and 10 isozymes from hemolysates. Similarly indices based on the electrophoretic zones identified genetic similarity only within the B group with respect to erythrocyte DT-diaphorase. Heterogeneity was detected among all groups with values higher in liver DT-diaphorase than in erythrocytes. Liver DTdiaphorase isozymes in groups C, D and E were significantly different from A and that of B from C. Grouping was based on cluster analysis of data on prothrombin in intraperitoneally injected rats, a parameter for resistance associated with DT-diaphorase.