HomePhilippine Journal of Veterinary Medicinevol. 44 no. 1 (2007)

Biochemical and Serological Identification of Mycoplasmas Isolated from Pneumonic Lungs of Slaughtered Cattle in Cotonou, Benin Republic

Razaki K. Adehan | Adebowale T.P Ajuwape | Adeyemi I. Adetosoye | Olugbenga O. Alaka

Discipline: Veterinary Medicine




Fifty bovine pneumonic lungs showing hard, dark lesion (consolidation) and motley appearance with or without fibrin on the pleural surface were collected from Cotonou, Republic of Benin and examined bacteriologically. Four Mycoplasma groups 48% (24/50) were isolated from the pneumonic lungs: 12 (50%) isolates which were tetrazolium-negative, phosphatase positive and digested serum, were serologically identified as M. mycoides subsp. mycoides SC (Group A). Seven (29.2%) isolates which hydrolyzed glucose and reduced tetrazolium chloride were identified serologically as M. bovirhinis (Group B). Three (12.5%) isolates which hydrolyzed glucose, reduced tetrazolium chloride and catabolized arginine were identified serologically as M. alvi(Group C). Two (8.3%) isolates which catabolized arginine were identified serologically as M. arginini (Group D). Other bacteria isolated were Staphylococcus aureus, S. epidermidis, Klebsiella species, Mannheimia (Pasteurella) haemolytica, Arcanobacterium (Actinomyces) pyogenes, Escherichia coli and Proteus vulgaris in order of occurrence. Histopathologically, the lungs showed typical fibrino-purulent lesions and severe alveolar collapse. CBPP is present in Cotonou, Benin Republic, hence sustained annual international vaccination campaign coupled with regular monitoring cannot be overemphasized, so that this mycoplasmal problem ravaging the cattle industry in Africa will not continue.