HomePhilippine Journal of Veterinary Medicinevol. 44 no. 1 (2007)

Pre-storage Semen Tests and Comparative Use of Skimmed Milk-Based Extender to Preserve Semen of Philippine Native and Anglo Nubian Goats

Orville L. Bondoc | Darrel Dane B. Serrano | Joseph F. Dela Cruz

Discipline: Veterinary Medicine

 

Abstract:

Semen from Philippine native and Anglo Nubian goats were initially evaluated using centrifugation test, cold shock treatment, and methylene blue reduction test. After centrifugation, the increase in sperm motility, mass activity, and percent live sperm after centrifugation was significantly higher in Philippine native than Anglo Nubian. Cold shock treatment resulted to lower sperm motility, mass activity, and percent live sperm. Reduction in sperm motility due to cold shock was higher in Philippine native than Anglo Nubian. Decrease in mass activity and percent live sperm was however, higher in Anglo Nubian than Philippine native. Reduction time of methylene blue dye was faster in Anglo Nubian than Philippine native, indicating better semen quality. Sperm motility and percent live sperm were significantly higher (P<0.05) for the skimmed milk-based extender than the egg yolk extender when stored at room (27°C) and refrigeration (5°C) temperatures. There was no significant difference (P>0.05) in motility and percent live sperm in extended semen of Philippine native and Anglo Nubian breeds. Based on a minimum motility rating of 40% and 30%, live sperm cells, storage time for extended semen under refrigeration temperature was slightly longer for Anglo Nubian (3 to 4 days) than Philippine native (2 to 3 days).