HomePhilippine Journal of Veterinary Medicinevol. 44 no. 2 (2007)

Seroprevalence of Campylobacter spp. Using Enzyme-Linked Immunosorbent Assay in Humans and Chickens from Selected Areas of Metro Manila and Laguna, Philippines

Poornima Manandhar | Loinda R. Baldrias | Asuncion K. Raymundo | Josefina C. Santos | Ma. Immaculada P. Torres | Lourdes M. Tapay | Raymond Tsang | Manuel M. Garcia

Discipline: Veterinary Medicine

 

Abstract:

An enzyme-linked immunosorbent assay (ELISA) was adopted to determine the occurrence of antibodies for Campylobacter jejuni/coli and C.jejuni O:19 in 128 human and 128 chicken serum samples. Seroprevalence to C.jejuni/coli among humans tested was found very high at 96.1% (123/128), compared to C. jejuni 0:19 at 0.6% (2/128). This is the first report on C. jejuni/coli seroprevalence in the country. Moreover, it is also the first report for serodiagnosis of C. jejuni 0:19 in the Philippines. The prevalence rates for antibodies to C. jejuni/coli and C.jejuni 0:19 among different age groups and between sexes were not significantly different (P < 0.05). Furthermore, there was also no difference in seroprevalence among exposed (chicken workers) and non-exposed (non-chicken workers) groups for C. jejuni/coli. However, the prevalence rate for C.jejuni 0:19 antibodies was higher (P<0.05) in the non-exposed group. In chickens, antibodies to C.jejuni/coli were also observed to be very high (P<0.01) at 80.5% (103/128) whereas, no antibodies to C.jejuni 0:19 was detected.