HomePhilippine Journal of Veterinary Medicinevol. 45 no. 1 (2008)

Seroprevalence of Neospora caninum in Bulgarian Murrah Buffaloes and itsDetection in Domestic Dogs from Buffalo Dairy Herds in Nueva Ecija, Philippines

Nancy S. Abes | Billy P. Divina

Discipline: Veterinary Medicine

 

Abstract:

The seroprevalence of Neospora caninum in female Bulgarian Murrah buffaloes in Nueva Ecija, relation between seropositivity and age groups (1-3 years, 4-6 years,>7 years) of the animals, and history of abortion, and its serological detection in dogs reared in close contact with seropositive buffaloes were studied. Competitive ELISA was used to test 176 buffalo sera from 36 dairy buffalo herds. IFAT was used for re-testing ELISA-positive samples and for sera-detection of the organism in dogs. Individual and herd-level prevalence based on cELISA were 27.3% ( 48/176) and 69.4% (25/36), respectively. IFAT detected 43 (90%) positives out of the 48 cELISA-positive samples. Prevalence seemed to increase with age, however, only age group >7 years (35.2%) had significantly higher prevalence than 1-3 years (17.4%) group. Analysis of 42 sera from seven herds showed no significant association between seropositivity and abortion history. However, an odds ratio of 1.5 was obtained suggesting that animals with history of abortion were 1.5 times more likely to become seropositive. As for 38 sera from dogs, IFAT detected 23 (60%) positives. Overall, this study showed the occurrence of antibodies to N caninum in Bulgarian Murrah buffaloes and dogs in the province of Nueva Ecija. It is recommended that further studies be done to determine the extent of the disease in buffaloes in the country and the mechanism of transmission within and among different buffalo herds.