Discipline: Veterinary Medicine
Helicobacters are spiral-shaped microbes with a number of recently identified species known to cause gastric ulceration, gastritis and gastric carcinoma in animals and humans. To demonstrate the occurrence of Helicobacter organisms in non-human host in the country, ten stray dogs from Northern Samar, Philippines were tested for the presence of Helicobacter species by invasive methods using urease test and brush cytology. Eight out of ten sampled animals consistently yielded positive urease activities Helicobacter like spiral organisms were discerned when examined by brush cytology. All the gastric sites of the fundic, cardiac and the pyloric regions yielded variably positive results. Positive samples were further tested and confirmed for Helicobacter spp. Identification using polymerase chain reaction method. Amplified DNA extracted yielded positively to Helicobacter spp. identification that corresponded to a 1,099 bp product. Current findings indicate that dogs may serve as sentinel for endemic presence of Helicobacter organisms that are of veterinary medical and public health importance. This study documented the presence of Helicobacter spp. in the stomach of an animal host in the country for the first time.