HomePhilippine Journal of Veterinary Medicinevol. 51 no. 1 (2014)

Enterotoxigenic and Attaching and Effacing Escherichia coli Strains in Diarrheic Piglets and Their Antibiogram

Swaraj Rajkhowa | Chandana Kalita | Dilip Kumar Sarma

Discipline: Veterinary Medicine

 

Abstract:

Enterotoxigenic Escherichia coli (ETEC) is a major cause of illness and death in neonatal and recently weaned pigs. Attaching and effacing Escherichia coli (AEEC) strains were also identified as a cause of diarrhea in pigs. A total of 157 Escherichia coli strains isolated from 137 pre-weaning and 101 post-weaning pigs with diarrhea were tested for the presence of genes representing ETEC and AEEC by PCR. Of these 157 isolates, 29.93% isolates were found to be ETEC and 2.54% as AEEC. Among the ETEC isolates, 48.93 % and 40.42 % were positive for genes est1 and elt1, respectively, and in 10.63% isolates both genes were present. Out of the four isolates that carried eaeA gene, none of the isolates was positive for genes like stx1, stx2e and bfpA. This is the first report of detection of AEEC in pigs from India. Most of the E. coli isolates exhibited resistance to streptomycin (95%), tetracycline (92%) and nalidixic acid (89%) and 69% of the isolates showed resistance to more than two antimicrobials. The present study throws light on the prevalence of ETEC and AEEC strains in diarrheic piglets and their antibiogram.