HomePhilippine Journal of Veterinary Medicinevol. 51 no. 1 (2014)

Subcutaneous Administration of L-carnitine is Associated with Differential Expression of Genes Involved in Glucose Uptake and Glycolysis in Iranian Baluchi Sheep (Ovis aries)

Mehdi Rashki Ghalehnoo | Ahmad Rashki

Discipline: Veterinary Medicine

 

Abstract:

 

The study was conducted to determine the effects of subcutaneous administration of L-carnitine on the expression of genes involved in fatty acid, glucose metabolism, glucose uptake and glycolysis in Iranian Baluchi sheep (Ovis aries). Twenty adult sheep were randomly divided into equal test and control groups and given subcutaneously 300 mg/75 kg of L-carnitine and 2 ml/75 kg distilled water daily for 28 days, respectively. After 28 days, the experimental and control sheep were all slaughtered for sampling; the total RNA was extracted from the liver samples using TRIzol Reagent method and changes in the transcript levels of selected genes were measured by RT-qPCR. The results showed that under this condition, the levels of expression of genes DQ174126, FBA1, FAB4, GPD1, GLUT1, GLUT3, G6PD, PFK1, PGK1, PRKAA1 and PRKAA2 encoding enzymes belonging to the glycolytic and lipogenic pathway in the liver were increased whereas HSDL1 and PCK1 were decreased by L-carnitine supplementation compared to the control. In conclusion, subcutaneously administered L-carnitine has beneficial effects on lipid and glucose homeostasis by inducing genes involved in glucose metabolism, fatty acid β-oxidation and glycolysis.