HomePhilippine Journal of Veterinary Medicinevol. 51 no. 2 (2014)

Isolation and Detection of Campylobacter jejuni from Ceca of Dressed Chickens of Commercial and Smallhold Raisers by Polymerase Chain Reaction

Loinda R. Baldrias | Susan A. Sedano | Manuel M. Garcia | Asuncion K. Raymundo

Discipline: Microbiology

 

Abstract:

The aim of this study is to recover putative Campylobacter spp. from cecal samples of chickens from commercial and smallhold raisers and to identify the Campylobacter jejuni isolates using polymerase chain reaction (PCR). C. jejuni was isolated from 125 cecal samples of chickens at dressing plants: 60 from commercial raisers and 65 from smallhold raisers. These were confirmed using PCR as C. jejuni by the formation of 402bp band with primers CL1 and CR2. Using the enrichment protocol, 4 (6.7%) C. jejuni isolates from 60 cecal samples of freshly dressed chickens from commercial producers were recovered. From smallhold raisers, one putative Campylobacter isolate (1.7%, 1/65) was isolated using enrichment protocol, while 14 isolates (70%, 14/20) were recovered by the application of the direct method (using three different selective agar media, without prior enrichment), of which 11/20 (55%) were identified as C. jejuni. The enrichment protocol and direct method with microaerobic environment conditions were used to recover the organism from the cecal samples. This study reports that C. jejuni is present in both commercial and smallhold raised chickens and that the direct method of isolation allowed for a higher rate of recovery compared to the enrichment method.