This research study determined the demographic profile of Level III nursing students of Colegio de San Juan de Letran Calamba and the different preventive health behaviors they practice. The study used descriptive comparative design. One hundred twenty-two (122) Level III nursing students were selected using random sampling method. A researcher’s self-constructed questionnaire was administered to the respondents and data were treated using relative frequency, weighted mean, and Chi-square test. It was concluded that the demographic variables of selected Level III nursing students affect the different preventive health behaviors that they perform. The study recommended conducting a deeper study to enhance and support what are presented in the questionnaire about preventive health behaviors on Influenza A (H1N1). The clinical instructors, on the other hand, should develop an educational program and teaching style to enhance students’ knowledge about preventive health behaviors on Influenza A (H1N1). Lastly, further studies should also be made to determine the preventive health behaviors among Level III nursing students when categorized with other variables such as marital status, self-efficacy, cognition, environment, and economic resources.