The study aimed to find out the relationship between aptitude test and preferences of elective subjects I and II of BSN Level II students of Letran Calamba, AY 2009-2010. It also aimed to determine the profile of the students in terms of age and gender and their aptitude test’s results in terms of verbal ability, numerical facility, and spatial perception; identify the preferences of the students in the elective subjects I and II; and determine the relationship of the profile of the students’ aptitude test and preferences in elective subjects I and II. The study is descriptive correlational and the data gathered were quantitative type. Frequency, percentage, and chi-square test were used as its statistical tools. It was found out that majority of the respondents are female and age 18 years old. Based on the aptitude test results, majority of them got failing grades in verbal ability but got very good grade in numerical ability while excellent in the spatial perception. It was also found out that the most preferred subject in elective I is Parent-Child nursing while Acute/Critical Care Nursing in elective II. In addition, it was proven that there is no significant difference between the aptitude test scores and preferences of the students in elective subjects I and II.