HomeInternational Rice Research Notesvol. 22 no. 2 (1997)

Anther Culture of Indica/Basmati Rice Heterotic F1 and F2 Hybrids and Selection of Desirable Double Haploid Lines

J S. Rohilla | J B. Chowdhury | N R. Yadav | V K. Chowdhury | R K. Jain | K R. Gupta

Discipline: Agriculture



Anther culture has been useful for the introgression of desirable traits into breeding populations of rice. We have been working on the transfer of dwarfness, defense against brown planthopper attack, and resistance to bacterial blight, from indica rice varieties to Basmati rice. This study included 12 indica rice varieties with one or more of the above mentioned desirable traits, six Basmati rice cultivars/breeding lines, and 31 heterotic F1/F2 hybrids obtained from different indica / Basmati crosses. Basmati rice parents included Basmati 370, Basmati 385, Pusa Basmati 1 (semidwarf), and advanced breeding lines (HBC5, HBC19, and HBC143) derived from Taraori Basmati. Panicles were collected from nethouse plants when the distance between flag leaf and penultimate leaf was 4-5 cm. Anthers were cultured in 9 cm petri dishes containing 30 ml N6 and MO19 media supplemented with either 2.0 mg NAAL-1, 1.0 mg kinetin L-1, and 0.5 mg 2,4-DL-1, or 1.0 mg kinetin L-1 and 2.0 mg 2,4-DL-1. MO19 is a modified MS medium which contains 3,134 mg KNO3L-1, 320 mg (NH4)2SO4L-1, 540 mg KH2PO4L-1 and lacks NH4NO3. The media contained 40 g sucrose L-1. Cultured anthers were subjected to a cold treatment at 10°C for 10 d and incubated under dark at 24+1°C. Data were recorded on percentage of anthers forming calli after 60 d. Microcalli at 2-4 mm diam were transferred to a modified MS medium containing 0.5 mg NAA L-1 and 1.0 mg each of BAP and kinetin L-1, kept under fluorescent light of 50 μE m-2s-1. Data were recorded on calli regenerating green and /or albino shoots after 30 d. Shoots were rooted and hardened in one-half strength MS medium supplemeted with 0.25 mg NAA L-1 and 2.5 mg multieffect triazole L-1 (MET, CNRRI, Hangzhou, China). All the media were semisolidified using 0.25% (w/v) phytagel (SIGMA, USA). Regenerated plants were transferred and grown to maturity in the nethouse.