HomeInternational Rice Research Notesvol. 22 no. 2 (1997)

Bioassay of Fusarium pallido- roseum (Cooke) Sacc., (Deutero-mycotina: Hyphomycetes) against leaffolder

S Manisegarane | S Letchoumanane | A Mohamed Hanifa | M Subramanian

Discipline: Agriculture



Greenhouse studies assessed the pathogenicity of Fusarium pallidoroseurn-on rice leaffolder (LF) Cnaphalocrosis medinalis Guenée at 28.5°C and between 88 and 90% relative humidity. The pathogen was tested at spore concentrations of 0-1×10 7 conidia mL-1. A stock solution of 1 mL was diluted with sterile distilled water, i.e., 100, 1000, etc., which meant 1 part of the fungus suspension was diluted in 99 or 999 parts of water. Thirty third-instar larvae were dipped for 2 to 3 s in each concentration. Treated larvae were transferred to filter paper and incubated in a petri dish containing fresh rice leaves. Mortality was determined after 1 wk of incubation and the percentage adjusted using Abbott's formula. Probit analysis showed that 5.49 × 103 conidia mL-1 was the effective concentration for LD50 of F. pallidoroseum.