HomeInternational Rice Research Notesvol. 23 no. 1 (1998)

Transferring wild abortive cytoplasmic male sterility through asymmetric fusion of protoplasts

B. Bhattacharjee | H. S. Gupta | A. P. Sane

Discipline: Agriculture, Genetics

 

Abstract:

We attempted a one-step transfer of wild abortive cytoplasmic male sterility (CMS) through asymmetric fusion between the protoplasts of CMS and fertile maintainer lines. We established embryogenic cell suspensions from the calli obtained from mature seed scu- tella of a CMS line (V20A), fertile maintainers (RCPL1-2C and V20B), and restorer IR36 lines. We isolated and cultured protoplasts to produce protocalli that differentiated into plants. Pollen grains of the protoplast-derived plants of the CMS line were sterile, whereas those derived from the maintainer and restorer lines were fertile.