The performance of various thermo- sensitive genic male sterile (TGMS) lines was assessed in order to use them in two-line intra- and intersubspecific hybridization with wide compatibility lines. Some 26 lines comprising TGMS lines, indica accessions, and japonica with wide compatibility (WC) gene(s) were used to make 65 hybrids: 31 were indica/indica and 34 were indica/japonica (WC). Standard heterosis was recorded up to 58%. Seed yield (g plant-1) ranged from 2.3 to 31.3. The number of heterotic combinations was higher in intersubspecific cross combinations. Spikelet fertility ranged from 2.7 to 88.4% in intersubspecific crosses and from 3.6 to 96.0% in intrasubspecific crosses. The WC lines were not univer- sally compatible in all combinations.