HomeInternational Rice Research Notesvol. 26 no. 2 (2001)

Adenine, adenosine monophosphate, and cytokinin: nutrients for rice seedling growth in the summer crop season

M.c. Hsieh | M.s. Tung

Discipline: Chemistry, Agriculture



Adenine, adenosine monophosphate (AMP), and cytokinin (isopentenyl adenine [IPA], transzeatin [t-Z], and dihydrozeatin [DHZ]) were tested to determine whether they could promote seedling growth of rice variety Tai-Keng 3 in Taiwan. Adenine and AMP are precursors of the biosynthesis of ATP, nucleic acids, coenzyme A, FAD NAD+, and cytokinin. Cytokinin is an inducer of protein synthesis and cell division. Rice seeds were surface-sterilized with 10% NaOCl for 10 min, rinsed with distilled water a few times, and placed under white fluorescent lamps (20 μmol m-2s-1) for germination at 26°C. Distilled water was replaced daily. Five-day-old germinating seeds were transferred to culture solutions, which included Hoagland solution [Ca(NO3)2, 5.0 × 10-3mol L-1; KNO3, 5.0 × 10-3 mol L-1; MgSO4, 2.0 × 10-3 mol L-1; KH2PO4 1.0 × 10-3 mol L-1; Fe EDTA, 1.18 × 10-5 mol L-1; and micronutrient solution consisting of H3BO3, 4.6 × 10-5 mol L-1; MnCl2 • 4 H2O, 9.14 × 10-6 mol L-1; ZnSO4 • 4 H2O, 7.65 × 10-7 mol L-1; CuSO4, 7.0 × 10-7 mol L-1; and H2MoO4, 1.24 × 10-7 mol L-1; pH 5.0; and in which adenine, AMP, IPA, t-Z, and DHZ were each added to make concentrations from 10-9 to 10-16 M].