Dark field photomicrography was used for the objective assessment of sperm motility in six water buffaloes-. The different movements of individual sperm cells from fresh and frozen semen were clearly observed and counted in the developed film. Abnormalities in sperm morphology and movements were likewise observed.
In this method, no significant difference on the percentage motility was observed between 1-and 2-sec exposures, and low and high power magnification. Better photomicrographs were obtained by using a lower magnification and a shorter exposure time.