The effects of electroacupuncture analgesia and local anesthesia for bloodless castration in water buffaloes were evaluated using pain response and behavior of the animals during and after the procedure. The electroacupuncture group responded with one good, three moderate and one poor analgesia while the lidocaine group showed moderate to good analgesia. The two acupoints used suggest that electroacupuncture produces analgesia but not sufficient for smooth conduct of the surgical operation. There was significant difference in the treatments usually observed between the control and electroacupuncture groups during regression two weeks (14 days) after and the rest of the monitoring days. The effects of local anesthesia and electroacupuncture analgesia are almost similar in terms of regression or healing rate. There was significant change in rectal temperatures before and after castration in local anesthesia and electroacupuncture analgesia.