An enzyme-linked immunosorbent assay (ELISA) was done to evaluate the antibody response of water buffaloes given autogenous hemorrhagic septicemia vaccine. Antibody rise using two types of vaccine preparation was compared: aluminum hydroxide (AIOH) absorbed, adjuvanted and double emulsion (DE) adjuvanted bacterins. Statistical analysis yielded a significant difference in
the antibody rise before and after administration of the two types of vaccine (P<0.05). Double emulsion adjuvanted vaccine yielded a significantly higher antibody response than the AIOH adsorbed adjuvanted vaccines (P<0.05). Passive mouse protection test (PMPT) significantly (P<0.05) resulted to a passing 80% protection rate of pooled sera (0.1100 - 0.1420 optical density) obtained from water buffaloes given AIOH adsorbed adjuvanted vaccines. Double emulsion (DE) adjuvanted vaccine obtained a 60% survival rate. Mice in the control group all died following inoculation of challenge organisms. Necropsied mice all yielded pure isolates of inoculated Pasteurella multocida (N. Samar strain G1).