Verification Studies on the Use of Biotech PCR-Based Detection Kit for Salmonella in Animal Feeds

Teresita  J. Ramirez,; Susan  A. Sedano,; Marie Antonette Ruth  V. Guerra,; Ma. Regina  C. Garcia,; Susana  M. Mercado,; Ma. Teresa  M. Perez,

Verification Studies on the Use of Biotech PCR-Based Detection Kit for Salmonella in Animal Feeds

Discipline: Veterinary Medicine, Animal Science

Abstract:

A detection kit employing polymerase chain reaction (PCR) technology was developed for Salmonella. Its application as Salmonella detection kit was investigated using different kinds of feeds, both in naturally contaminated and artificially spiked feed samples. The kit was compared and validated against standard/traditional culbJral assay involving enrichment step and plating in selective media. The BIOTECH Salmonella detection protocol included short enrichment period that allowed multiplication of Salmonella, DNA extraction and amplification of DNA In a thennal cyder followed by a simple gel electrophoresis procedure.
Both the conventional and the BIOTECH PCR-based kit can detect the microorganism In Salmonella-inoculated feed samples, with 1000/0 agreement value. Slightly lower agreement value (960/0) was obtained on natural-contaminated samples due to the low number of Salmonella cells present even after enrichment period. Standard cultural method required 5-7 days to confirm the presence and identity of Salmonella, while the PCR-based kit reduced the detection time to less than 28 hrs.