The utilization of vitrified bovine oocytes as recipient cytoplasts for intraspecies and intergeneric somatic cell nuclear transfer (NT) was investigated. In vitro matured bovine metaphase II (Mil) oocytes freed of cumulus cells were vitrified either in microtips with a vitrification solution (VS) containing 10% ethylene glycol (EG), 10% dimethyl sulfoxide (DMSO) and 0.6 M sucrose or in microdrops with another VS containing 35% EG, 5% polyvinyl-pyrrolidone and 0.4 M trehalose. Oocytes vitrified in microdrops showed a higher survival rate than those oocytes vitrified in microtips. The cleavage and development to the blastocyst stage of the NT embryos constructed with cumulus cells and vitrified oocytes in microdrops were similar to those of non-vitrified control. The cell numbers of blastocysts derived from NT embryos constructed with vitrified recipient oocytes were lower than those of the non-vitrified control, regardless of the vitrification procedure. The rates of fusion, cleavage and development to the blastocyst stage between the intraspecies and intergeneric
somatic NT groupswere similar. Intergeneric NT embryos formed blastocoel earlier than in intraspecies somatic NT embryos, but, with fewer blastocyst cell numbers. In conclusion, the result demonstrates the production of swamp buffalo somatic cell NT embryos using vitrified bovine oocytes as recipient cytoplasts.