HomePhilippine Journal of Veterinary and Animal Sciencesvol. 31 no. 1 (2005)

Genetic Diversity within and Among Populations of Philippine Mallard Ducks (Anas Platyrhynchos L.) Detected Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Arnolfo M. Monleon | Angel L. Lambio | A. A. Barrion | Ninfa P. Roxas

Discipline: Animal Science



The five Philippine mallard duck (Anas platyrhynchos L.) populations, namely: Cagayan Valley, Central Luzon, Northern Mindanao, Southern Tagalog and Western Visayas were investigated for genetic diversity. Ten samples of genomic DNA from each of the five populations were used. Ten random primers were utilized in the amplification process. The primers yielding amplification products (bands) were used in screening all the DNA samples. The RAPD data were obtained using binary system and analyzed in the popgene software (Popgene version 1.31). The one-way analysis of variance determined the level of significance. A total of 70 loci were detected from the A. platyrhynchos L. using nine primers. About 76% of loci were polymorphic per population and 100% polymorphic among populations. The gene diversity within population ranged from 0.17±0.16 (Cagayan Valley) to 0.24±0.16 (Central Luzon) and 0.25±0.16 among population. The estimate of genetic distance was lower (0.08±0.01) and showed closer relationship between comparisons of population (P>O.O5). The dendrogram showed that the geographically close populations had more similar genetic relationship than those that were farther apart. The survey populations of A. platyrhynchos were deduced to follow the non-equilibrium model. These findings provide genetic basis on the reported variations within and among populations as well as on the origin of lineage of the Philippine mallard ducks.