Blood from pure (Philippine Carabao, Murrah and Bulgarian Murrah) and crossbreed (PC x Bulgarian Murrah and PC X Murrah) water buffaloes of different sex and age, stages of growth, pregnancy and lactation were analyzed and compared for their plasma glucose, cholesterol, triglyceride, total protein and uric acid levels, using a BTS-310 photometer. ANOVA was carried in CRD with unequal number of observations. The result showed no difference in plasma cholesterol and total protein of caracalves between male and female one year old caracalves, while male caracalves had higher (P<0.05) triglyceride and uric acid and lower plasma glucose in caracalves at this age. At two years old, only plasma total protein was observed to be higher in female than in male buffaloes. Pregnancy did not affect the plasma glucose and uric acid values of female buffaloes. Plasma total protein increased with advancing stage of pregnancy. Plasma cholesterol tended to decrease as pregnancy progressed. Plasma triglyceride was elevated in dry pregnant buffaloes at 2nd trimester. Lactation tended to increase plasma cholesterol and total protein and decrease triglyceride values. The glucose, cholesterol, triglyceride and total protein levels for the different age and physiologic states were highest in one year old and below, lactating, mature non pregnant lactating and 13 months to two year old buffalo, respectively, while uric acid did not differ among physiologic states and age.