Medium chain fatty acid is a phytochemical constituent found in the coconut oil and this study focused on the blood glucose lowering potential of formulated freshly prepared and marketed coconut (Cocos nucifera) oil. In the acute oral toxicity dose none of the test animals died and displayed signs of toxicity after administration of the fixed doses of 300mg/kg, 2000mg/kg and 5000mg/kg of freshly prepared coconut oil. The Approximate Effective Dose (AED) showed that the potential of the plant as a hypoglycemic agent after the tested animals showed a decreased in blood glucose level after four hours of administration of freshly prepared and marketed coconut oil. Statistical analysis using two-way ANOVA is quite necessary to determine if decrease in glucose level (post induction) is significant. Tukey’s Multiple Comparison test was used to determine which of the four treatments and monitoring time showed a significant decrease in glucose level of the test animals. Comparing these least significant ranges with the differences in ordered means, the researcher arrived at the conclusion that glibenclamide as positive control was significantly different from the formulated emulsion of coconut oil. It followed also that the mean value of the negative control was significantly different from the formulated emulsion of coconut oil. This implied that glibenclamide as positive control was most effective in lowering the blood glucose level of hyperglycemic-induced rabbits, followed by formulated emulsion, marketed coconut oil and lastly by the placebo.