Philippine E-Journals

Antioxidant Activity of Lyophilized Crude Extracts from the Leaves of Bixaorellana, Centrosemapubescens, Clerodendrumspeciosum, Cyanthilliumcinereum and Syzygiumaqueum

Jovy A. Dulay

 

Abstract:

This study aimed to determine the total flavonoids, total phenolic content and antioxidant activity of frozen dried samples of Bixaorellana, Centrosemapubescens, Clerodendrumspeciosum, Cyanthilliumcinereum and Syzygiumaqueum. Standard laboratory procedures using the UV-Vis spectrophotometer was used to determine the test parameters. Based from the data, it was found that total flavonoids as quercetin (ppm) were substantially higher particularly on Bixaorellana, Centrosemapubescens, Clerodendrumspeciosum, Cyanthilliumcinereum and Syzygiumaqueum. Moreover, Clerodendrumspeciosum, Syzygiumaqueum, and Centrosemapubescens had slightly higher total phenolic content as garlic acid (ppm) compared to Cyanthilliumcinereum and Bixaorellana. The median inhibitory concentrations (IC50) were obtained and tested for antioxidant activity. Results of the test proved that there was an existing significant difference on the antioxidant properties of the various plant extracts. Of these, positive control (ascorbic acid) was the most potent antioxidant agent. Based on the findings, Clerodendrumspeciosum and Centrosemapubescens were the most potent antioxidant plant materials compared to Bixaorellana, Cyanthilliumcinereum and Syzygiumaqueum.