The primary purpose of this quantitative study was to examine and determine the relationship between organizational culture in terms of the four organizational dimensions (mission, adaptability, involvement and consistency) and chemical laboratory safety and security and compliance (laboratory environment; emergency equipment and planning; personal protective equipment, signs, labels, plans and postings; electrical hazards; storage; compressed gases and cryogenics; chemical and waste disposal; chemical hoods and ventilation; security and training and awareness) among Higher Education Institutions (HEIs) in Davao City. The research used the descriptive survey method using validated research questionnaire in the conduct of the research study with 50 respondents from five institutions during the second semester of school year 2017-2018. The statistical tools used in this study were descriptive statistics and Pearson Product-Moment Correlation. Findings revealed the great extent of the organizational culture of the respondent HEIs. Also, the status of chemical laboratory safety and security and compliance of Higher Education Institutions in Davao City implies that the data gathered were relevant and valid since the data point tend to be very close to the mean. Using the Pearson Product-Moment Correlation, results revealed a significant low to moderate correlation between organizational culture and chemical laboratory safety and security and compliance.