Philippine E-Journals

Antiangiogenic Activity and Bioremediation Capacity of Formulated Tea from Kalanchoepinnata (“Katakataka”) and Colocasiaesculenta (“Taro”) Leaves

Reysa Raphaelle G. Gabilagon

 

Abstract:

The study was conducted to investigate the antiangiogenic activity and biosorption capacity of formulated tea from Kalanchoepinnata (Katakataka) and Colocasiaesculenta (Taro) leaves. This study utilized the Chorioallantoic membrane assay in duck embryos in determining the antiangiogenic activity of the plant materials. The biosorption capacity of the formulated tea on lead (Pb) and cadmium (Cd) was tested using the Atomic Absorption Spectrophotometer (AAS). The acute oral toxicity dose was tested in Swiss albino mice according to the OECD Guidelines. The research instruments used were Scanning Electron Microscope (SEM) for the determination of the factors affecting the biosorption; Fourier-Transfer Infrared Spectrometer was used to determine the functional groups present in the plant material; DPPH assay in determining the Median Inhibitory Concentration (IC50) and antioxidant activity; and microbial limit and heavy metal content determination using AAS was conducted to ensure the quality of the formulated tea. Results of the experiment showed that Kalanchoepinnata (Katakataka) and Colocasiaesculenta (Taro) were practically non-toxic, potent antioxidant and antiangiogenic agents. Statistical analysis showed that the Kalanchoepinnata (Katakataka) leaves ethanolic extract was more effective in reducing the blood vessels formation in the treated duck embryos. Both plants were also observed as potent bioremediating agent as shown in the reduction of lead and cadmium content of the reacting solutions.