Hyperlipidemia causes arteriosclerosis which is a risk factor for coronary heart disease. The problem can be due to hereditary factors, but commonly it is an acquired condition resulting from unhealthy diet and lifestyle. Globally, diseases of the heart and stroke made it among the top three causes of mortality. In the Philippines, 21 percent of deaths were due to cardiovascular diseases. In this regard, the researcher used an experimental research for the investigation of banana (Musa x paradisiaca L.) peel extract on its fat hydrolyzing and lowering of blood cholesterol characteristics. In order to measure the activity of purified HMG-CoA Reductase Activity on Musa x paradisiaca L. peel extract, an enzymatic assay was used using Abcam HMG-CoA Reductase Activity Assay. The potential of Musa x paradisiaca L. as a fat hydrolyzing agent was determined by the researcher using the procedure on “Estimation of Saponification Value of Fats”. To determine if there was a significant difference on the cholesterol lowering activity and fat hydrolysis potential of Musa x paradisiaca L. peel extracts, the statistical tools used were mean, standard deviation, and analysis of variance (ANOVA). The Musa x paradisiaca L. peel extract showed a potential in hydrolyzing fats which was similar to the result in the HMG-CoA Reductase Activity of the plant extract at 50 ppm. This proved that the positive control atorvastatin (10 mM) and the plant extract (50 ppm) had a mean difference of -49.574, showed an existing significant difference on its mean inhibitory activity. The peel extract was therefore a potent inhibitor of HMG-CoA reductase. This study showed that the fat hydrolyzing potential of Musa x paradisiaca L. peel extract was capable of lowering the levels of blood cholesterol. The Musa x paradisiaca L. peel extract at 50 ppm concentrations showed the closest activity in the action potential of atorvastatin, 10mM.