HomeTin-awvol. 2 no. 1 (2018)

Antiangiogenic Effect of Silver Nanoparticles Synthesized from Turmeric (Curcuma longa) Rhizome and Chloroform Fraction on Chicken Chorioallantoic Membrane

Ian Ace A. Pacquiao



Cancer is a disease that severely impacts a patient’s quality of life. The aim of the research was to determine the antiangiogenic effect of the chloroform turmeric (Curcuma longa) and synthesized silver nanoparticles. The research employed true experimental design. The study made use of phytochemical screening, thin layer chromatography, Fourier Transform Infrared Spectroscopy, UV-Vis- Spectrophotometry, Scanning Electron Microscopy, Median Lethal Dose and Median Effective Dose Determination as well as Chicken Chorioallantoic Membrane Assay. The research employed one way analysis of variance (ANOVA) for the parameters for determination of degree of angiogenesis and Post Hoc Tukey HSD Multiple Comparisons test was performed to determine which treatment groups had a significant difference. Phytochemical screening and TLC yielded positive results to polyphenols, tannins, flavonoids, anthraquinones, terpenoids, steroids and coumarins. Characterization of silver nanoparticles via UV-Vis Spectrophotometry indicated spherical nanoparticles with a Surface Plasmon Resonance of 415 nanometers and confirmed by Scanning Electron Microscopy. Median Lethal Dose for turmeric and silver nanoparticles were 83.559 mg/kg and 123.07 mg/kg, respectively. Median effective dose for turmeric and silver nanoparticles were 42.7174 mg/kg and 84.00 mg/kg, respectively. ANOVA results indicated that all five treatments had a significant difference (p<0.05). Post Hoc Tukey HSD Multiple Comparisons test indicated that the positive control, turmeric and silver nanoparticles had significant difference against the negative control and the control group, whereas the three treatments were statistically comparable.