HomeScientific Augustinianvol. 9 no. 1 (2019)

Hemostatic Activity of Chitosan from Squid Cuttlebone and Mud Crab Shell

Jenalyn Faith D. Caras | Jose G. Perez | Carmel Anne Brasales | Kent Devin Bangonon | Sheena Joy Barredo | Wilcent John Celis | Louie Bryn Dela Cruz | Jun Rey Paller | Thyrealle Frances Sorongon | Cherry Dee Teñales



Excessive bleeding is one of the factors of unsuccessful or traumatic surgery and one of the leading causes of death. Dressings based on chitin and chitosan are the most promising hemostatic agents due to their notable properties such as anti-bacterial and regeneration stimulant. Squid’s cuttlebone is a biological waste that can be an alternative source of chitosan aside from mud crabs. The study aimed to compare the hemostatic activity of mud crab shell and squid cuttlebone chitosan extracts. It also intended to determine if there is a significant difference between untreated blood and blood treated with these extracts by performing coagulation studies. Chitin was extracted from the shell of mud crab and squid cuttlebone and converted to chitosan. Twenty (20) untreated human blood samples were tested for Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT). After which, a post-test on the same samples treated with 10 µL of chitosan extracts was conducted. No significant difference was observed in the PT and PTT of human blood and other samples treated with mud crab shell and squid cuttlebone. Results showed that the squid cuttlebone chitosan extract has a comparable hemostatic activity with mud crab shell and can be an alternative for treating bleeding tendencies.