Philippine E-Journals

Antibacterial Synergism of Amoxicillin and Mabolo (Diospyros blancoi) Leaf Extracts

Jose G. Perez | Jenalyn Faith D. Caras | Ma. Consuelo Martina S Daza | Yocha Belle P Armendares | Adrienne Krizty S Batino | Steven G Caya | Hannah Marie Angelic Doromal | Aldrin Jules F Galboso | Kathleen Chris G Lapac | Barby Ann P Pedregosa

 

Abstract:

Studies have shown that one of the leading causes of deaths these days is bacterial resistance to antibiotics. Nowadays, scientists combine antibiotics with other enhancing components such as plant extracts acting in synergy towards killing bacteria. One plant that possesses considerable potential is Mabolo (Diospyros blancoi) (velvet apple) the leaves of which contains phytochemicals that can act as an antibacterial agent against common pathogens. Hence, this study aimed to determine the antibacterial synergy of the crude methanolic and aqueous extracts of mabolo leaf and amoxicillin against S. aureus and E. coli by combining leaf extracts with Amoxicillin. Antibacterial activity was tested using Agar Well Method, and zones of inhibition were measured using a caliper. Post-test only control group experimental research design was used to identify the significant difference at 0.01 α. The mabolo crude methanolic and aqueous extracts produced zones of inhibition against S. aureus. Treatments with the highest concentration of the crude methanolic and aqueous extracts of mabolo (LME1000A and LAE1000A) with Amoxicillin had wider zones of inhibition compared with treatments with Amoxicillin alone, and are comparable to the zones of inhibition produced by Co-amoxiclav. These results indicate that S. aureus is sensitive to Amoxicillin and that methanolic and aqueous extracts of mabolo leaves have antibacterial activity against S. aureus. In addition, the wider zones of inhibition formed by the combinations of Amoxicillin and leaf extracts suggest a synergistic effect of the 2 antibacterial preparations against S. aureus. On the other hand, neither Amoxicillin, the methanolic and aqueous extracts of mabolo leaves nor combinations of Amoxicillin and the extracts formed zones of inhibition against E. coli. These results suggest that E. coli is resistant to Amoxicillin and the crude methanolic and aqueous extracts of mabolo leaves do not have antibacterial activity against E. coli.