Home⇛SARIRIT: The University Research Journal (formerly QSU Research Journal)⇛vol. 13 no. 1 (2024)

Potential Of Aloe Vera Leaf Extract (Avle) For In-Situ Production Of Latundan Meriplants

Krisette Maie J Sagales | Henrey R. Ignacio | Romelyn S. Tugade | Elizabeth R. Oppuer | Nigel Fernand T. Corpuz | Elizabeth T. Carig

Discipline: agricultural sciences

 

Abstract:

Plant extracts are natural source of growth regulators that are costeffective and environmentally friendly. This study was conducted to evaluate the effect of various Aloe Vera Leaf Extract (AVLE) concentrations (ppm) as an alternative rooting hormone for the in-situ production of TCB meriplants. The study comprised of five treatments: T0- control IBA, T1- with 20ppm of AVLE, T2- with 30ppm of AVLE, T3- with 40ppm of AVLE, and T4- with 50ppm of AVLE. The study followed a Completely Randomized Design (CRD) and each treatment was replicated three times with five meriplants present within each growing vessel. Corms of experimental meriplants with three or more leaves were cleaned for the rooting phase. Data collection began a week after the meriplants were placed in the growth vessel with the required level of AVLE as rooting medium and continued until the fourth week of culture. Data include the number of roots, root length, and number of leaves. This process is repeated weekly until the meriplants are ready for ex-situ hardening stage on the fourth week. Results showed that the treatment with the highest number of roots, root length, and number of leaves was observed in T1 (20ppm of AVLE) with 88% survival rate. Based on the findings, it can be concluded that AVLE showed comparable performance to control treatment (IBA) in rooting of tissue cultured banana. Hence, it can be recommended to utilize T1 (20ppm of AVLE) for the rooting stage of Latundan



References:

  1. Amissah, N., Monney, M. A. D., & Blay, E. (2016). Influence of BA and IBA or NAA combinations on micropropagation of Cryptolepis sanguinolenta. American Journal of Plant Sciences, 7(3), 423–430. https://doi.org/10.4236/ajps.2016.73050
  2. Atkinson, J. A., Rasmussen, A., & Traini, R. (2014). Branching out in roots: Uncovering form, function, and regulation. Plant Physiology, 166(2), 538–550. https://doi.org/10.1104/pp.114.245423
  3. Basak, U., Das, A., & Das, P. (2000). Rooting response in stem cuttings from five species of mangrove trees: Effect of auxins and enzyme activities. Marine Biology, 136(2), 185–189. https://doi.org/10.1007/s002270050021
  4. Belniaki, A. C., Das, L. A., Rabel, N., Gomes, E. N., & Zufellato-Ribas, C. K. (2018). Presence of leaves on Coleus stem cuttings influences their rooting. Ornamental Horticulture, 24(3), 206–210. https://doi.org/10.14295/oh.v24i3.1204
  5. Berjak, P., & Pammenter, N. W. (2017). Encyclopedia of applied plant sciences (2nd ed.). Academic Press.
  6. Cutler, H. G., & Schneider, B. A. (1990). Plant growth regulator handbook. Plant Growth Regulator Society of America.
  7. Dagne, E., Bisrat, D., Viljoen, A., & Van Wyk, B. E. (2000). Chemistry of Aloe species. Current Organic Chemistry, 4(10), 1055–1078. https://doi.org/10.2174/1385272003375932
  8. Dias, M. I., Sousa, M. J., Alves, R. C., & Ferreira, I. (2016). Exploring plant tissue culture to improve the production of phenolic compounds: A review. Industrial Crops and Products, 82, 9–22. https://doi.org/10.1016/j.indcrop.2015.12.016
  9. Dobrescu, A., Giuffrida, M. V., & Tsaftaris, S. A. (2017). Leveraging multiple datasets for deep leaf counting. Proceedings of the IEEE International Conference on Computer Vision Workshops (ICCVW), 2072–2079. https://doi.org/10.1109/ICCVW.2017.243
  10. Dunsin, O., Ajiboye, G., & Adeyemo, T. (2016). Effect of alternative hormones on the rootability of Parkia biglobosa. Scientia Agriculturae, 13(2), 113–118. https://doi.org/10.15192/PSCP.SA.2016.13.2.113118
  11. El Sherif, F. (2017). Aloe vera leaf extract as a potential growth enhancer for Populus trees grown under in vitro conditions. American Journal of Plant Biology, 2(4), 101–105. https://doi.org/10.11648/j.ajpb.20170204.11
  12. El-Shereif, N. A. (2018). Impact of plant tissue culture on agricultural sustainability of agricultural environment in Egypt: Part II. In The handbook of environmental chemistry (Vol. 77). Springer. https://doi.org/10.1007/698_2017_160
  13. Galavi, M., Karimian, M. A., & Mousavi, S. R. (2013). Effects of different auxin (IBA) concentrations and planting beds on rooting grape cuttings (Vitis vinifera). Annual Review & Research in Biology, 3(4), 517–523.
  14. Hamouda, A. M. A., Hendi, D. M. G., & Abu-El-Leel, O. F. A. (2012). Improving basil growth, yield and oil production by Aloe vera extract and active dry yeast. Egyptian Journal of Horticulture, 39(1), 45–71.
  15. Hilty, J., Muller, B., Pantin, F., & Leuzinger, S. (2021). Plant growth: The what, the how, and the why. New Phytologist, 232(2), 25–40. https://doi.org/10.1111/nph.17610
  16. Ku, Y. S., Sintaha, M., Cheung, M. Y., & Lam, H. M. (2018). Plant hormone signaling crosstalks between biotic and abiotic stress responses. International Journal of Molecular Sciences, 19(10), 3206. https://doi.org/10.3390/ijms19103206
  17. Liang, C., Wu, R., Han, Y., Wan, T., & Cai, Y. (2019). Optimizing suitable antibiotics for bacterium control in micropropagation of cherry rootstock using a modified leaf disk diffusion method and E test. Plants, 8(3), 66. https://doi.org/10.3390/plants8030066
  18. Mady, A. (2009). Effect of certain medicinal plant extracts on growth, yield and metabolism of some medicinal aromatic plants (Unpublished master’s thesis). Al-Azhar University.
  19. Méndez-Hernández, H. A., Ledezma-Rodríguez, M., Avilez-Montalvo, R. N., Juárez-Gómez, Y. L., Skeete, A., & Avilez-Montalvo, J. (2019). Signaling overview of plant somatic embryogenesis. Frontiers in Plant Science, 10, 77. https://doi.org/10.3389/fpls.2019.00077
  20. Mitra, A., Singh, M., Banga, A., Pandey, J., Tripathi, S., & Singh, D. (2022). Bioactive compounds and therapeutic properties of Aloe vera: A review. Plant Science Today, 10(2), 1–7. https://doi.org/10.14719/pst.1715
  21. Muttulani, M. A. (2022). Grape (Vitis vinifera L.) propagation using different types of cuttings and root-initiating substances. Journal of Agricultural Research, Development, Extension and Technology, 4(1), 1–9.
  22. Ngomuo, M., Mneney, E., & Ndakidemi, P. A. (2014). The in vitro propagation techniques for producing banana using shoot tip cultures. American Journal of Plant Sciences, 5(11), 1614–1622. https://doi.org/10.4236/ajps.2014.511175
  23. Orlikowska, T., Nowak, K., & Reed, B. (2017). Bacteria in the plant tissue culture environment. Plant Cell, Tissue and Organ Culture, 128(3), 487–508. https://doi.org/10.1007/s11240-016-1144-9
  24. Pierre-Jerome, E., Drapek, C., & Benfey, P. N. (2018). Regulation of division and differentiation of plant stem cells. Annual Review of Cell and Developmental Biology, 34(1), 289–310. https://doi.org/10.1146/annurev-cellbio-100617-062459
  25. Pour, M. M., Saberi-Riseh, R., Mohammadinejad, R., & Hossein, A. (2019). Nano-encapsulation of plant growth promoting rhizobacteria and their metabolites using alginate-silica nanoparticles and carbon nanotube improves UCB1 pistachio micropropagation. Journal of Microbiology and Biotechnology, 29(7), 1096–1103. https://doi.org/10.4014/jmb.1903.03022
  26. Sahu, K. P., Giri, D. D., Singh, R., Pandey, P., Gupta, S., Shrivastava, A. K., Kumar, A., & Pandey, D. K. (2013). Effect of Aloe vera on some annual plants. Pharmacology & Pharmacy, 4(7), 599–610. https://doi.org/10.4236/pp.2013.47086
  27. Shidiki, A. A., Ambebe, T. F., & Mendi, A. G. (2019). A comparative evaluation of indole-3-butyric acid and plant extracts as potential rooting enhancers in cuttings of Vitex diversifolia and Cordia millenii. International Journal of Forest, Animal and Fisheries Research, 3(4), 1–6.
  28. Shigenaga, A. M., & Argueso, C. T. (2016). No hormone to rule them all: Interactions of plant hormones during the responses of plants to pathogens. Seminars in Cell & Developmental Biology, 56, 174–189. https://doi.org/10.1016/j.semcdb.2016.06.005
  29. Suman, S. (2017). Plant tissue culture: A promising tool of quality material production with special reference to micropropagation of banana. Biochemistry and Cellular Archives, 17(1), 1–26.
  30. Surjushe, A., Vasani, R., & Saple, D. (2008). Aloe vera: A short review. Indian Journal of Dermatology, 53(4), 163–166. https://doi.org/10.4103/0019-5154.44785
  31. Ullah, A., Manghwar, H., Shaban, M., Khan, A. H., Akbar, A., & Ali, U. (2018). Phytohormones enhanced drought tolerance in plants: A coping strategy. Environmental Science and Pollution Research, 25(33), 33103–33118. https://doi.org/10.1007/s11356-018-3364-5
  32. University of Florida. (n.d.). Micropropagation (types and stages). In PropG Tissue Culture (IFAS). https://propg.ifas.ufl.edu/09-tissue-culture/01-types/04-tctypes-micropropagation.html
  33. Walter, A., & Schurr, U. (1999). The modular character of growth in Nicotiana tabacum plants under steady-state nutrition. Journal of Experimental Botany, 50(336), 1169–1177. https://doi.org/10.1093/jxb/50.336.1169

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