The study aimed to develop and validate a final examination for the course “Principles of Food Safety, Hygiene and Sanitation”. The items of the examination were based on the course syllabus and the table of specifications developed by the researchers. The exam was administered to the first year students as a final examination at the end of the semester. They were given two (2) hours to finish the exam. After checking the exam, the papers were divided into high and low group, specifically, the top 25 % and low 25%. Both groups consisted of 75 students each. Item analysis which includes item difficulty and item discrimination was done. The test items are normally distributed according to level of difficulty with most of the items classified as moderately difficult. There are equal distributions of items according to the discrimination levels of low, moderate and high. Although there are 38 items that have been identified to be poor, it is still advised that the test items be revised or the options be reviewed so that they could be translated into good items.